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Hipure Plasmid Ef MIDI Kit (Spin) Plasmid DNA1
Chemical ReagentCell Transfection Animal InjectionFluorescent DNA Sequencing
Magen Biotechnology (Guangzhou) Co., Ltd.
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Product Description Product DescriptionHiPure Plasmid EF Midi Kit (Spin)CAT NOPRODUCT NAMESIZEPRICEP123102HiPure Plasmid EF Midi Kit(Vacuum)50 preps$423.00P123102BHiPure Plasmid EF Midi Kit(Spin)50 preps$547.00△ IntroductionThe HiPure Midi system provides a fast, simple, and cost-effective plasmid DNA midiprep method for routine molecular biology laboratory applications. HiPure Midiprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries.Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.△ Details FeaturesSpecificationsMain FunctionsIsolation up to 500μg endotoxin-free plasmid DNA from 25-50ml bacterial cultureApplicationsCell transfection, animal injection, etc.Purification methodMidi spin columnPurification technologySilica technologyProcess methodManual (centrifugation or vacuum)Sample typeConventional plasmid, plasmid less than 30KBSample amount25-50mlYield10-500μgElution volume≥0.4mlTime per run≤60 minutesLiquid carrying volume per column4mlBinding yield of column250μg △ AdvantagesHigh purity - purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.Fast - silica gel column purification is much faster than ion exchangeHigh yield - up to 250μg plasmid can be binded in one columnLow endotoxin content - the obtained plasmid can be directly used in cell transfection and animal injection△ Kit ContentsContentsP123102P123102BPurification Times50 Preps50 PrepsRNase A30 mg30 mgBuffer E1150 ml150 mlBuffer E2150 ml150 mlBuffer E3150 ml150 mlBuffer E4150 ml150 mlBuffer E5150 ml150 mlBuffer PW2*50 ml50 mlElution Buffer30 ml30 mlMaxPure EF Mini Column50502 ml Collection Tubes5050Lysate Clear Midi Syringe5050Extend Tubes505050ml Centrifuge Tubes 50Support Tubes 50△ Storage and StabilityThe kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37oC to dissolve. After addition of RNase A and optional LyseBlue reagent, Buffer P1 is stable for 6 months when stored at 2-8°C. Company Profile Exhibition all over the world Packaging & Shipping Certificate Laboratory Workshop Fininshed product area
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