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Hot selling in Europe and America HiPure FFPE DNA Kit1
Chemical ReagentHiPure FFPE DNA KitHiPure FFPE DNA Kit FFPE block
Magen Biotechnology (Guangzhou) Co., Ltd.
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Product Description Product DescriptionHiPure FFPE DNA Kit CAT NOPRODUCT NAMESIZEPRICED312602HiPure FFPE DNA Kit50 preps$124.00D312603HiPure FFPE DNA Kit250 preps$497.00HiPure FFPE Nucleic acid Kit supplies a simple and rapid DNA extraction for formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 20 minutes (not including digestion time). DNA can be directly used for downstream applications such as PCR, southern blot and viral DNA detection, etc.SpecificationsFeaturesSpecificationsMain FunctionsIsolation total DNA from FFPE tissue samplesApplicationsPCR, southern blot and viral DNA detection, etc.Purification methodMini spin columnPurification technologySilica technologyProcess methodManual (centrifugation or vacuum)Sample typeFormalin-fixed, paraffin-embedded (FFPE) tissue and sections samplesSample amount<20mgElution volume>15μlTime per run≤20 minutesLiquid carrying volume per column4mlBinding yield of column100μgPrincipleHipure FFPE Nuclear acid kit adopts silica gel column purification. The sample is deparaffinated by xylene and digested by lysate and protease. After de crosslinked at 90 oC, DNA/RNA is released into the lysate. Adding ethanol to adjust the binding conditions, the sample is transferred to the column where DNA/RNA is adsorbed on the membrane and protein is removed without adsorption. Protein and other impurities are washed by buffer GW1, and the salt is removed by buffer GW2. Finally, the DNA / RNA is eluted by low salt buffer.AdvantagesSafety - deparaffinating without contacting with xylene or other toxic solutionFast - without overnight incubation and digestion, several samples can be extracted within 2hoursHigh efficiency -remove the formaldehyde modification of DNA, greatly enhancing the sensitivity of PCRKit ContentsContentsD312602D312603Purification Times50 Preps250 PrepsHiPure DNA Mini Columns I502502ml Collection Tubes50250Buffer DPS30 ml150 mlBuffer ATL15 ml60 mlBuffer AL15 ml60 mlBuffer GW1*22 ml88 mlBuffer GW2*12 ml50 mlProteinase K24 mg120 mgProtease Dissolve Buffer1.8 ml10 mlBuffer AE10 ml30 mlStorage and StabilityProteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used. Experiment Data Detailed Photos Protocol Company Profile FAQ Q1.What is the Trade Term?A1:Ex-work factory (Guangzhou)Q2. How long is the guarantee (period)?A2:The product can be stored properly for at least 18 months .Q3.Are you a manufacturer ? A3:Yes, we were established in 2012 and our headquarter is in Guangzhou. All products can be provided with OEM packaging, greatly reduce the cost, and can be flexible in technology and other aspects of communication and cooperation.Q4.How long is our Production leading time?A4:Generally our lead time is 2-7 working days after receiving 100% prepayment. If order in bulk , lead time will be longer. Customized bulk quantities are determined by the specific quantity.Q5.What is the Payment term?A5:T/T, Paypal, Alipay, Western Union. 100% payment in advance.Q6.What is the packing details?A6:The outer packaging of general products is cardboard boxes. Low-temperature products will have ice packs places in foam boxes and then packed into cartons.Q7. What support you will have to assemble this extractor?A7: Our extraction instrument is integrated, easy to operate and does not require assembly.Q8.What kind of documents we will provide to you?A8:B/L or AWB number, Commercial Invoice, Packing List, MSDS, COA.Q9. How to apply the free samples?A9: For free samples we have to know your company name, your E-mail, your FedEx account, and the detail address of your company include the phone number. You can contact with us to apply the free samples.Q10. Do you accept the small orders?A10: Yes, we accept the small orders. We will try our best to support our customer's business whether the small trail order.
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